About
The Jasco J-1500 Circular Dichroism Spectrophotometer is an instrument designed for analytical spectroscopy for analyzing chirality in molecules through their optical activity. Circular dichroism (CD) measures the difference in absorption of left- and right-handed circularly polarized light. This technique is often used to determine the secondary structure of peptides and proteins. A temperature control system is coupled with a six-cell holder to run thermal melts which can be analyzed for thermodynamic and kinetic data sets for conformational and folding studies of peptides and proteins. The wavelength range for the instrument is extensive, which can obtain measurements in both the vacuum-ultraviolet and near infrared spectral regions using the standard photomultiplier tube detector (163 – 950 nm).
Instrument Location
Ammon Pinizzotto Biopharmaceutical Innovation Center
590 Avenue 1743, 2nd Floor, Room 244
Newark, DE 19713
Note: Swipe access to the building, floor, and lab are required.
Instrument Contacts
Primary Contact: Albree Weisen (albree@udel.edu)
Secondary Contact: Sirui Shen (sirius@udel.edu)
Training Requirements
In order to use the Jasco J-1500 Circular Dichroism Spectrophotometer, users must complete online training. The following should be completed in order:
- Read Standard Operating Procedures
- Watch Training Video
- Complete and Pass Training Quiz
Supplementary Information
The following supplementary material is available for those who would like more information about the system. Additionally, feel free to check out the Circular Dichroism (CD) page where there are scientific resources available as well.
The following page provides information on R scripts that can be used for automatic data analysis of CD data:
Training Video
This video goes over the basics of circular dichroism spectroscopy and details the standard operating procedures for the Jasco J-1500 Circular Dichroism Spectrophotometer. Users must watch this video before taking the quiz and getting access to the instrument.