Reversed-Phase High Performance Liquid Chromatography (RP-HPLC)

Reversed-Phase High Performance Liquid Chromatography (RP-HPLC) is a purification method that is commonly used for the separation of many different types of compounds due to its reproducibility and broad applicability. Many researchers use RP-HPLC for isolation of target peptides from organic scavengers, terminated sequences, deletion sequences, and chemically modified sequences from side reactions during either the synthesis or the cleavage of the peptide. Below are some scientific articles that can aid those that would like to become more familiar with the technique, as well as provide a variety of method alterations that can be made for improved separation of peptides from impurities. The most general, and often useful, resources are listed first. For those that want to learn more, the training video for our Teledyne ISCO ACCQPrep HP150 HPLC Preparative System may be useful as well. The introduction also has a great overview of RP-HPLC.

HPLC of Peptides and Proteins: Methods and Protocols

Marie-Isabel Aguilar

Springer, 3-22 (2004).

DOI: 10.1385/1-59259-742-4:3

Peptide Characterization and Application Protocols

Gregg B. Fields

Springer, 3-55 (2007).

DOI: 10.1007/978-1-59745-430-8_1

Analysis of Peptides by High-Performance Liquid Chromatography

Colin T. Mant, Robert S. Hodges

Methods Enzymol., 271 3-50 (1996).

DOI: 10.1016/S0076-6879(96)71003-0

High-Resolution Reversed-Phase High-Performance Liquid Chromatography of Peptides and Proteins

Marie-Isabel Aguilar, Milton T.W. Hearn

Methods Enzymol., 270 3-26 (1996).

DOI: 10.1016/S0076-6879(96)70003-4

Effects of Ion-Pairing Reagents on the Prediction of Peptide Retention in Reversed-Phase High-Resolution Liquid Chromatography

Dacheng Guo, Colin T. Mant, Robert S. Hodges

J. Chromatogr. A, 386 205-222 (1987).

DOI: 10.1016/S0021-9673(01)94598-4

Prediction of Peptide Retention Times in Reversed-Phase High-Performance Liquid Chromatography I. Determination of Retention Coefficients of Amino Acid Residues of Model Synthetic Peptides

Dacheng Guo, Colin T. Mant, Ashok K. Taneja, J. M. Robert Parker, Robert S. Hodges

J. Chromatogr. A, 359 499-518 (1986).

DOI: 10.1016/0021-9673(86)80102-9

The Isolation of Peptides by High-Performance Liquid Chromatography Using Predicted Elution Positions

C. A. Browne, H. P. J. Bennet, S. Solomon

Anal. Biochem., 124 201-208 (1982).

DOI: 10.1016/0003-2697(82)90238-X