About
The Teledyne ISCO ACCQPrep HP150 High Performance Liquid Chromatography Preparative System features easy-to-use software with programmable gradients, UV detection and peak collection, and automatic detection of collection tube racks for peptide purification. It handles flow rates from 1-150 mL/min, with up to 6000 psi capability for operation of columns at high flow rates for maximum throughput and added tolerance for high back pressures as columns age. A two-component gradient can be formed from up to six solvents. The system includes active solvent level sensing and detection of a full waste container. This system, coupled to an AutoSampler, includes four fraction collection racks and one sample rack for single or repeated injections of multiple samples. Two Waters XBridge 30 mm (internal diameter) by 250 mm (length) C18 columns are attached to the system: one for acidic solvents and one for basic solvents. A Phenomenex 21.2 mm (internal diameter) by 250 mm (length) C18 column is also attached for neutral solvents.
Instrument Location
Ammon Pinizzotto Biopharmaceutical Innovation Center
590 Avenue 1743, 2nd Floor, Room 255
Newark, DE 19713
Note: Swipe access to the building, floor, and lab are required.
Instrument Contacts
Primary Contact: Aditya Natu (adinatu@udel.edu)
Secondary Contact: Jacob Schwartz (schwartj@udel.edu)
Training Requirements
In order to schedule use of the Teledyne ISCO ACCQPrep HP150 High Performance Liquid Chromatography Preparative System, users must complete online training. The following should be completed in order:
- Read Standard Operating Procedures
- Watch Training Video
- Complete and Pass Training Quiz
Supplementary Material
The following supplementary material is available for those who would like more information about the system. Feel free to visit the Reversed-Phase High Performance Liquid Chromatography (RP-HPLC) page where there are scientific resources available as well.
Training Video
This video goes over the basics of reversed-phase high performance liquid chromatography and details the standard operating procedures for the Teledyne ISCO ACCQPrep High Performance Liquid Chromatography Preparative System. Users must watch this video before taking the quiz and getting access to the instrument.